BOX 6 Molecular markers and marker-assisted selection for pearl millet in India Pearl millet is a cereal grown for foodgrain and straw in the hottest, driest areas of Africa and Asia where rainfed and dryland agriculture are practised. It is similar to maize in its breeding behaviour. Traditional farmers' varieties are open-pollinated and out-breeding and thus continuously changing.
Iran J Pharm Res. Due to the increasing number of GMOs imported to Iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs.
In this study, following the extraction of genomic DNA from processed foods sold commercially in Iran, qualitative PCR was performed to detect genetically modified maize.
Based on the gel electrophoresis results, Bt- 11 and MON events were detected in some maize samples, while, in none of them Bt- modified gene was detected. For the first time, the results demonstrate the presence of genetically modified maize in Iranian food products, reinforcing the need for the development of labeling system and valid quantitative methods in routine analyses.
Introduction Genetic modification has been an extremely valuable tool in plant genetic research. The genetically modified organism GMO contains an exogenous gene which is able to express a new protein that confers new traits, i.
Many genetically modified crops have been approved worldwide since mids and the global area of these crops has expanded yearly since their commercialization.
Inmillion hectares were cultivated with genetically modified crops mainly soybean, maize, cotton and canola 34. Therefore, the detection and identification of GMOs in food have become important issues for all the subjects involved in food control 9 Qualitative testing may be used to discriminate between the authorized and unauthorized GM food to identify safe or potentially unsafe material 11 Although several analytical methods have been proposed, GMO detection methods generally involve specific DNA sequence detection by means of PCR techniques, able to detect even small amounts of transgenes in raw materials and processed foods 13 In this study, we examined the detection of recombinant DNA of genetically modified maize in several processed foodstuffs gathered from Iranian market.
This method includes the following steps: From reference material Appropriate reference materials for positive and negative controls provide the basis for the validation of analytical procedures The following commercially available Certified Reference Materials were used: From processed food samples In total 25 food samples including a variety of processed material, from relatively mild treated maize to highly processed products containing maize ingredients, for human consumption were gathered randomly from different Iranian supermarkets.
The analyzed products were as follows: Oligonucleotide primers Oligonucleotide primers were purchased as purified and desalted specimen from Eurofins.
The sequences of oligonucleotide primers are given in Table 1.Sep 17, · In this paper, DNA extraction methods have been evaluated to detect the presence of genetically modified organisms (GMOs) in maize food and feed products commercialised in Turkey. All the extraction methods tested performed well for the majority of maize foods and feed products analysed.
Eventspecific detection of stacked genetically modified maize Bt11×GA21 by UP-M-PCR and real-time PCR.
J. Agr. Food Chem. – () CrossRef Google Scholar Rudi K, Rud I, Holck A. Read chapter 7 Future Genetic-Engineering Technologies: Genetically engineered (GE) crops were first introduced commercially in the s.
After two decad. Genetically modified crops (GMCs, GM crops, or biotech crops) are plants used in agriculture, the DNA of which has been modified using genetic engineering methods. In most cases, the aim is to introduce a new trait to the plant which does not occur naturally in the species.
Examples in food crops include resistance to certain pests, diseases, or environmental conditions, reduction of spoilage.
The foodproof SL GMO MON Maize Detection Kit enables qualitative detection of genetically modified maize MON by real-time PCR. The cultivation of maize MON and its use in food and feed are allowed in the EU since However, some EU countries have imposed a national ban since , such as France and Germany.
In , the global cultivation area of genetically modified (gm) crops increased to million hectares (estimation) worldwide (James, ).
Besides gm soybean, gm .